Research Associate I, AAV Biology

About AskBio

Asklepios BioPharmaceutical, Inc. (AskBio) is a leading, clinical-stage gene therapy company founded in 2001 based on the work of adeno-associated virus (AAV) gene therapy visionary and pioneer, Jude Samulski, PhD. Since that time, our commitment to developing life-saving medicines that can potentially cure genetic diseases has never wavered.

At AskBio, we’ve built a foundation of therapeutic programs, AAV manufacturing and a rich portfolio of patented technology. Our therapeutic focus includes neuromuscular, central nervous system, cardiovascular and metabolic diseases with clinical trials underway for Pompe disease, Parkinson’s disease, and congestive heart failure. Our gene therapy platform includes Pro10™, an industry-leading proprietary cell line manufacturing process, and an extensive AAV capsid and promoter library.

We became a wholly-owned, independently operated, subsidiary of Bayer AG in late 2020 as a cornerstone of its newly formed Cell & Gene Therapy (CGT) platform. Our global headquarters is in Research Triangle Park, NC. Additionally, we have significant operations in Philadelphia, PA, Columbus, OH, Scotland, France, and Spain.

Our vision: Pioneering science to create transformative molecular medicines.

Our mission: Lead innovative science and drive clinical outcomes to transform people’s lives.

Our principles:

  • Advance innovative science by pushing boundaries.
  • Bring transformative therapeutics to patients in need. 
  • Provide an environment for employees to reach their fullest potential. 

Our values:

  • Be a Pioneer. We are not afraid of the impossible and to innovate to make gene therapies accessible to those in need.
  • Cultivate Collaboration. Strive to be the best teammate, actively listen, openly communicate, and embrace diverse points of view.
  • Embrace Responsibility. We are humbled by the enormity of our mission. We hold a relentless commitment to advance science and clinical outcomes for our patients, families, and caregivers.
  • Raise the Bar. Continuously drive improvements and efficiencies. Seek and provide constructive feedback. Have a bias for learning and action.
  • Act with Uncompromising Integrity. Be honest, transparent, and committed to doing what’s right in every situation. Make clear commitments and follow through.

We have an opportunity for a Research Associate, AAV Biology to join the AskBio team in our Research Triangle Park (Durham), NC location and be part of our continued success!

Position Overview 

The Research Associate, AAV Biology, reporting to the Scientist, will conduct experimental bench work in support of development of AAV based Gene Therapy clinical candidate vectors as part of the R&D department. The ideal candidate will have direct experience with quantitative molecular biology techniques, including plasmid cloning, isolation of DNA, RNA, and protein from cells and tissues, quantitative PCR, RT-PCR, ELISA, SDS-PAGE, and Western Blot. The location of this position is RTP (Durham), North Carolina.

Responsibilities

  • Hands-on experiences in plasmid purification, genomic DNA extraction, and RNA extraction.
  • Experience in molecular cloning, including restriction enzyme digestion, ligation, DNA assembly, transformation, etc.
  • Perform in vitro assays, cell culture, animal tissue handling.
  • Perform and evaluate data analysis at completion of each test method; Summarize analytical results and final reports.
  • Ensure lab areas are clean, organized and equipment, supplies and raw materials are available for use.
  • Contribute to study reports, study protocols, SOPs, and regulatory filings technical discussions and investigations.

Required Education and Experience

  • BS degree in Molecular Biology, Biochemistry or other relevant degree with 1 – 3 years research experience
  • Hands-on Experiences in molecular biology techniques such as: plasmid cloning, DNA/RNA isolation, SDS-PAGE, and Western blot.

Preferred Experience and Skills

  • Prior experience in cell line maintenance.
  • Advanced technical lab skills with the ability to assume independent ownership of protocols/experiments
  • Ability to multitask among multiple projects and teams and work independently in a fast-paced, highly interactive environment.
  • Knowledge implementing and troubleshooting experiments.
  • Self-motivated, organized, capable of working independently and in a collaborative environment with cross-disciplinary teams.
  • Excellent verbal and written communication skills with the ability to maintain formal recording.
  • Proficiency in Microsoft Office Suite (Word, Excel, PowerPoint). Experience in the SnapGene and GraphPad.
  • Understanding of statistics and data analysis/interpretation.
  • Good judgment and innovation to achieve a solution within standard procedures.
  • AAV background.

Asklepios BioPharmaceutical, Inc. (AskBio) is an Equal Opportunity Employer and does not discriminate against any employee or applicant for employment because of race, color, religion, gender, sexual orientation, gender identity, national origin, age, disability, veteran status or any other protected status prohibited under Federal, State or local laws. All employment decisions are based on valid job‐related requirements. If you are a qualified individual with a disability or a disabled veteran and are unable or limited in your ability to use or access our website, you may request a reasonable accommodation to express interest in a specific opening by calling us at (919) 561-6210 or emailing us at talent@askbio.com.